Our Clinical Laboratories fulfill all your HLA Testing needs with the leading HLA typing technology, rapid turnaround times and the utmost attention to quality.
Sirona Dx laboratory provids the fastest turnaround time and best price for HLA typing to research scientists, academic investigators and Contract Research Organizations (CRO). We work with you to achieve your goal within your budget and timeline.
HLA-typing is inevitably needed for many types of research on T cell immunology, all flow cytometric work on HLA multimers and most functional cellular assays such as ELISpot. Correlating the immune response of the subjects with their HLA type is a critical step in success of carrying out clinical trials for vaccine and vaccine development efforts.
HLA alleles are associated with several diseases and they are implicated in many types of immune response. Determination of a subject's HLA type may reveal critical information in ascertaining drug efficacy or outcome of a disease investigation. Some HLA alleles are known to modulate the immune response of a patient to certain therapeutic products that may lead to heightened drug susceptibility. HLA typing of subjects is the key to establishing preventative measures to such responses and pursuing other treatment options.
Two levels of HLA Testing services to fit your needs:
- Level 1: Intermediate Resolution HLA Testing by SSOP
- Level 2: High Resolution HLA Testing by sequencing (SBT) or SSP
Level 1: Intermediate Resolution HLA Testing by SSOP
Intermediate resolution HLA typing results include a subset of alleles sharing the digits in the first field of their allele name. This resolves major allele groups to 4 digits, with some degeneracy such as: A*02:01/02:02/02:05/02:36.
- PCR-SSOP hybridization is used to determine HLA typing at an intermediate level of resolution. The methodology combines locus-specific amplification of genomic DNA by PCR and hybridization with sequence-specific oligonucleotide probes (SSOP). The probes are designed to match nucleotide sequences at polymorphic sites of exons 2 and 3 for HLA alleles. The genotypes for these loci are assigned by their unique hybridization patterns.
Level 2: High Resolution HLA Testing by SBT and SSP
High resolution HLA typing resolves alleles to a specific 4-digit allele, with occasional degeneracy that results in a set of alleles specifying and encoding the same protein sequence for the peptide binding region of an HLA molecule.
- Formulations of allele or group specific primer sets are used to amplify genomic DNA. Setup includes mixing a reaction buffer with human genomic DNA sample and Taq DNA Polymerase, dispensing the mixture to the PCR tubes, and thermal cycling. Once cycling is complete, the PCR products are loaded onto an agarose gel for electrophoresis. Following electrophoresis, gel is photographed and interpreted for the specific amplification patterns.
- Sequence Based Typing (SBT) directly identifies DNA sequence from target HLA genes. DNA amplification is performed using locus specific primers accompanied by cycling amplification buffers, Taq DNA polymerase and sample genomic DNA. The resulting product is treated and cycle sequenced. The final reactions are purified, denatured and loaded on an automated sequencing instrument for detection. The nucleotide chronology and resulting HLA subtype is determined by multicolor, fluorescence-based sequencing.
We have consistently achieved the turnaround-time and accuracy needed by our clients at peak efficiency. We currently offer the following turn-around-times:
HLA Loci Routinely Tested:
Within the MHC region, the laboratory routinely types classical Class I and Class II loci as presented in table below:
|Class I||Class II|
Sample Types and Order Handling:
Sirona Dx offers testing on various sample types that have been validated at the laboratory. These include fresh or frozen blood, genomic DNA, buccal swabs, cryopreserved cells and tissues.