Menu

Spatial Biology

Nanostring CosMx

Sirona Dx is the first CRO to provide access to the latest spatial multiomics 3D imaging technology from Nanostring.

Our CosMx spatial imaging service utilizes the CosMx platform to achieve multiomic, multiplex, subcellular resolution with high sensitivity from any tissue sample type.

Capabilities

  • Multiomic: RNA + protein detection
  • Multiplex: 1000 RNAs, 68 proteins
  • Subcellular: ~50 nm resolution in x, y plane
  • Sensitivity: 1-2 copies RNA per cell, multiple probes/gene
  • Samples: diverse types including FFPE, fresh frozen, TMA and organoids

Content

  • 1000-Plex RNA Human Universal Cell Characterization Panel
    • cell typing, cell-cell interaction analysis, and more in a wide range of human tissues and disease states, + up to 50 custom targets
  • 100-Plex RNA Human Immuno-oncology Panel
    • cell typing of key immune and stromal cells and evaluation of immune infiltration and tumor microenvironments, + up to 10 custom targets
  • 1000-Plex RNA Mouse Neuroscience Panel
    • cell typing, cell-cell interaction analysis, and more in mouse brain and other neuronal tissues, + up to 10 custom targets
  • 68-Plex Protein Human Protein Assays
    • cell profiling of immune and non-immune cells, including T cells, B cells, macrophages, NK cells, epithelia and stroma
CosMx-Analysis
CosMx-Analysis

Our CosMx spatial imaging service utilizes the CosMx platform to achieve multiomic, multiplex, subcellular resolution with high sensitivity from any tissue sample type.

Capabilities

  • Multiomic: RNA + protein detection
  • Multiplex: 1000 RNAs, 68 proteins
  • Subcellular: ~50 nm resolution in x, y plane
  • Sensitivity: 1-2 copies RNA per cell, multiple probes/gene
  • Samples: diverse types including FFPE, fresh frozen, TMA and organoids

Content

  • 1000-Plex RNA Human Universal Cell Characterization Panel
    • cell typing, cell-cell interaction analysis, and more in a wide range of human tissues and disease states, + up to 50 custom targets
  • 100-Plex RNA Human Immuno-oncology Panel
    • cell typing of key immune and stromal cells and evaluation of immune infiltration and tumor microenvironments, + up to 10 custom targets
  • 1000-Plex RNA Mouse Neuroscience Panel
    • cell typing, cell-cell interaction analysis, and more in mouse brain and other neuronal tissues, + up to 10 custom targets
  • 68-Plex Protein Human Protein Assays
    • cell profiling of immune and non-immune cells, including T cells, B cells, macrophages, NK cells, epithelia and stroma
CosMx-Analysis2

Comprehensive End to End Service Includes:

  • Assay customization
  • Custom conjugation & verification
  • Multiplex Assay Optimization
  • Slide Staining
  • Advanced Bioinformatics Analysis

Applications

  • Biomarker discovery and development
  • Immuno-oncology
  • Inflammatory and autoimmune diseases
  • Neuro-immunology
  • Characterization of tissue microenvironment
  • Therapeutic response
  • Drug safety and toxicity
  • Oncology

Please reach us at [email protected] for more information

Fluidigm Hyperion

The Hyperion™ Imaging Mass Cytometry (IMC) is based on laser ablation technology coupled with time-of-flight (TOF) mass cytometry. This technology allows for a comprehensive understanding of complex cellular phenotypes and their interrelationships in the spatial context of the tissue microenvironment.

Sirona Dx pioneered Imaging Mass Cytometry™ applications for biopharma launching the world’s first commercial CRO service in 2018. A leading-edge technology, IMC enables highly multiplexed immunohistochemistry (mIHC) with simultaneous detection of up to 40 protein markers in the same tissue section with subcellular, single cell resolution.

Capabilities

  • Multiplexing:  40 markers
  • Dynamic Range: 4 orders of magnitude
  • Resolution: 1um = 1 image pixel
  • Advantage: large panels, distinct signals with low crossover, single tube multi-sample multi-plex, simple panel reconfigurations
  • Assay Support: broad array of commercially available antibodies as well as proprietary antibodies, amenable to formalin-fixed, paraffin-embedded or frozen tissue sections

Content

  • Maxpar panel kits
    • Human
      • Immune activation
      • Immuno-oncology
      • Tissue architecture
      • Tumor infiltrating lymphocytes
    • Mouse
      • tissue architecture
      • cancer cell process
      • immune activation
      • immune phenotyping
    • Customization
      • Antibody conjugation
      • Panel design
hyperion
hyperion

Sirona Dx pioneered Imaging Mass Cytometry™ applications for biopharma launching the world’s first commercial CRO service in 2018. A leading-edge technology, IMC enables highly multiplexed immunohistochemistry (mIHC) with simultaneous detection of up to 40 protein markers in the same tissue section with subcellular, single cell resolution.

Capabilities

  • Multiplexing:  40 markers
  • Dynamic Range: 4 orders of magnitude
  • Resolution: 1um = 1 image pixel
  • Advantage: large panels, distinct signals with low crossover, single tube multi-sample multi-plex, simple panel reconfigurations
  • Assay Support: broad array of commercially available antibodies as well as proprietary antibodies, amenable to formalin-fixed, paraffin-embedded or frozen tissue sections

Content

  • Maxpar panel kits
    • Human
      • Immune activation
      • Immuno-oncology
      • Tissue architecture
      • Tumor infiltrating lymphocytes
    • Mouse
      • tissue architecture
      • cancer cell process
      • immune activation
      • immune phenotyping
    • Customization
      • Antibody conjugation
      • Panel design
hyperion3
hyperion2

Comprehensive End to End Service Includes:

  • Assay design (40 markers)
  • Custom Ab Conjugation & Verification
  • Multiplex Assay Optimization
  • Slide Staining
  • Laser Ablation & Image Acquisition
  • Advanced Bioinformatics Analysis

IMC Applications

  • Biomarker discovery
  • Cell segmentation
  • Immuno-oncology
  • Infectious disease
  • Phenotyping

Frequently Asked Questions

What sample types can be profiled by IMC?

Suitable biological sample types include frozen or formalin-fixed, paraffin-embedded (FFPE) tissue sections or cell smears (liquid biopsies) deposited onto glass slides.

What is the resolution of IMC?

1 µm. The IMC process directs a 200 Hz laser focused at 1 µm to tissues stained with metal-tagged antibodies and directs these metal tags to analysis by inductively coupled plasma time-of-flight (TOF) technology, the basis of CyTOF® mass cytometers. A discrete signal from each ionized metal tag is detected based on differences in mass instead of wavelength, and at 1 Da resolution with minimal background.

What Antibodies are available for IMC?

Since 2018, we have developed a large inventory of metal tagged IMC antibodies. Some are purchased directly from Fluidigm but many more are conjugated at our laboratory using metal labelling kits manufactured by Fluidigm. Our inventory is continuously expanding. If we don’t stock your marker/clone we can custom conjugate. Please contact us for more details.

Please reach us at [email protected] for more information

Lunaphore COMET

Sirona Dx is the first CRO in North America to provide access to the COMET™ platform from Lunaphore.

Our industry leading spatial biology service suite now includes the COMET™ platform to perform hyperplex assays based on flexible and fully automated sequential immunofluorescence (seqIF™) to develop custom panels.

Capabilities

  • Throughput – high capacity (4 slides) and rapid turnaround time (40 minutes every two markers) provide unmatched hyperplex throughput.
  • Fast assay development: panels can be developed in weeks rather than months. Panels are optimized by characterizing simple modules of 1-2 markers. Once characterized, markers can be shuffled in any protocol cycle or added to any new panel without any further optimization. Batch optimization possible (Figure 2).
  • Hyperplex: automated 40-plex runs detected on the same tissue slide.
  • Reproducibility – full automation, use of standard primary antibodies without the need of complex upstream conjugation and direct detection via secondary antibodies, eliminate downstream complexity and variability leading to high reproducibility.
  • Quality – highly controlled staining conditions thanks to a microfluidic technology (FFeX™) deliver immunofluorescence multiplex analysis comparable to classic IHC.
  • Preserved tissue morphology – low exposure to reagents thanks to rapid incubations, and a gentle antibody removal, preserve the tissue to obtain high-quality stainings and enable downstream applications and H&E cross-validation on the same sample.
  • Off-the-shelf primary antibodies – with a reagent agnostic approach, optimize using your own clones and reagent library; no conjugation and further validation required.
  • Subcellular resolution – 230 nm resolution
  • Integrated microscope – 20X magnification, TRITC, Cy5, DAPI channels.
  • Common sample types: FFPE, FS, TMAs
  • Output OME.TIFF – file supported by most image analysis tools.

Content

lunaphore-comet-front-new
lunaphore-comet-front-new

Our industry leading spatial biology service suite now includes the COMET™ platform to perform hyperplex assays based on flexible and fully automated sequential immunofluorescence (seqIF™) to develop custom panels.

Capabilities

  • Throughput – high capacity (4 slides) and rapid turnaround time (40 minutes every two markers) provide unmatched hyperplex throughput.
  • Fast assay development: panels can be developed in weeks rather than months. Panels are optimized by characterizing simple modules of 1-2 markers. Once characterized, markers can be shuffled in any protocol cycle or added to any new panel without any further optimization. Batch optimization possible (Figure 2).
  • Hyperplex: automated 40-plex runs detected on the same tissue slide.
  • Reproducibility – full automation, use of standard primary antibodies without the need of complex upstream conjugation and direct detection via secondary antibodies, eliminate downstream complexity and variability leading to high reproducibility.
  • Quality – highly controlled staining conditions thanks to a microfluidic technology (FFeX™) deliver immunofluorescence multiplex analysis comparable to classic IHC.
  • Preserved tissue morphology – low exposure to reagents thanks to rapid incubations, and a gentle antibody removal, preserve the tissue to obtain high-quality stainings and enable downstream applications and H&E cross-validation on the same sample.
  • Off-the-shelf primary antibodies – with a reagent agnostic approach, optimize using your own clones and reagent library; no conjugation and further validation required.
  • Subcellular resolution – 230 nm resolution
  • Integrated microscope – 20X magnification, TRITC, Cy5, DAPI channels.
  • Common sample types: FFPE, FS, TMAs
  • Output OME.TIFF – file supported by most image analysis tools.

Content

Building seqIF panels

Figure 1. Hyperplex panels can be easily developed on COMET™ with guided protocols for optimization and flexible choice of marker positioning.

COMET single-cell resolution

Figure 3. Single-cell resolution achieved with 40-plex staining on COMET™. The left panel shows five markers detected on a TMA of lung adenocarcinoma with lymph node metastasis. The middle and right panels show a selection of regions of interest (ROIs) to identify different cell types.

Akoya CODEX

Comprehensive End to End Service Includes:

  • Assay customization
  • Multiplex Assay Optimization
  • Slide Staining
  • Advanced Bioinformatics Analysis

Applications

  • Biomarker discovery and development
  • Immuno-oncology
  • Inflammatory and autoimmune diseases
  • Neuro-immunology
  • Characterization of tissue microenvironment
  • Therapeutic response
  • Drug safety and toxicity
  • Oncology

Please reach us at [email protected] for more information

The Akoya CODEX/Phenocycler ™ platform provides high parameter spatial analysis in tissues of interest.

Our Akoya tissue profiling capability utilizes CODEX/PhenocyclerTM to localize 100+ proteins and RNA at single cell resolution to better understand cell-cell interactions and localization within the tissue microenvironment.

Capabilities

  • Unbiased: Whole slide imaging at single cell resolution
  • Ultrahigh-plex: Image 100+ protein and RNA biomarkers
  • Multiomic: Analyze protein and RNA biomarkers in situ
  • Utilizes oligonucleotide tag barcodes and dye oligonucleotides reporters through sequential hybridization/dehybridization cycling

Content

  • PhenoCode Discovery Immune Profiling Human Protein Core
PhenoCycler
PhenoCycler

Our Akoya tissue profiling capability utilizes CODEX/PhenocyclerTM to localize 100+ proteins and RNA at single cell resolution to better understand cell-cell interactions and localization within the tissue microenvironment.

Capabilities

  • Unbiased: Whole slide imaging at single cell resolution
  • Ultrahigh-plex: Image 100+ protein and RNA biomarkers
  • Multiomic: Analyze protein and RNA biomarkers in situ
  • Utilizes oligonucleotide tag barcodes and dye oligonucleotides reporters through sequential hybridization/dehybridization cycling

Content

  • PhenoCode Discovery Immune Profiling Human Protein Core

Comprehensive End to End Service Includes:

  • Assay customization
  • Multiplex Assay Optimization
  • Bioinformatics Analysis

Applications

  • Immuno-oncology
  • Infectious disease
  • Inflammatory diseases
  • Immunoprofiling
  • Drug discovery

Please reach us at [email protected] for more information

Contact Us

Email: [email protected]
Phone: (503) 816-9692

Address

4640 Southwest Macadam Avenue,
Portland, Oregon 97239

Hours

Monday – Friday: 8:00am – 6:00pm PST
Saturday – Sunday: Closed