Fluidigm Hyperion

The Hyperion^™ Imaging Mass Cytometry (IMC) is based on laser ablation technology coupled with time-of-flight (TOF) mass cytometry. This technology allows for a comprehensive understanding of complex cellular phenotypes and their interrelationships in the spatial context of the tissue microenvironment.

Sirona Dx pioneered Imaging Mass Cytometry™ applications for biopharma launching the world’s first commercial CRO service in 2018. A leading-edge technology, IMC enables highly multiplexed immunohistochemistry (mIHC) with simultaneous detection of up to 40 protein markers in the same tissue section with subcellular, single cell resolution.

Capabilities

Multiplexing: 40 markers
Dynamic Range: 4 orders of magnitude
Resolution: 1um = 1 image pixel
Advantage: large panels, distinct signals with low crossover, single tube multi-sample multi-plex, simple panel reconfigurations
Assay Support: broad array of commercially available antibodies as well as proprietary antibodies, amenable to formalin-fixed, paraffin-embedded or frozen tissue sections

Content

Maxpar panel kits
- Human
  - Immune activation
  - Immuno-oncology
  - Tissue architecture
  - Tumor infiltrating lymphocytes
- Mouse
  - tissue architecture
  - cancer cell process
  - immune activation
  - immune phenotyping
- Customization
  - Antibody conjugation
  - Panel design

Sirona Dx pioneered Imaging Mass Cytometry^™ applications for biopharma launching the world’s first commercial CRO service in 2018. A leading-edge technology, IMC enables highly multiplexed immunohistochemistry (mIHC) with simultaneous detection of up to 40 protein markers in the same tissue section with subcellular, single cell resolution.

Capabilities

Multiplexing: 40 markers
Dynamic Range: 4 orders of magnitude
Resolution: 1um = 1 image pixel
Advantage: large panels, distinct signals with low crossover, single tube multi-sample multi-plex, simple panel reconfigurations
Assay Support: broad array of commercially available antibodies as well as proprietary antibodies, amenable to formalin-fixed, paraffin-embedded or frozen tissue sections

Content

Maxpar panel kits
- Human
  - Immune activation
  - Immuno-oncology
  - Tissue architecture
  - Tumor infiltrating lymphocytes
- Mouse
  - tissue architecture
  - cancer cell process
  - immune activation
  - immune phenotyping
- Customization
  - Antibody conjugation
  - Panel design

Comprehensive End to End Service Includes:

Assay design (40 markers)
Custom Ab Conjugation & Verification
Multiplex Assay Optimization
Slide Staining
Laser Ablation & Image Acquisition
Advanced Bioinformatics Analysis

IMC Applications

Biomarker discovery
Cell segmentation
Immuno-oncology
Infectious disease
Phenotyping

Frequently Asked Questions

What sample types can be profiled by IMC?

Suitable biological sample types include frozen or formalin-fixed, paraffin-embedded (FFPE) tissue sections or cell smears (liquid biopsies) deposited onto glass slides.

What is the resolution of IMC?

1 µm. The IMC process directs a 200 Hz laser focused at 1 µm to tissues stained with metal-tagged antibodies and directs these metal tags to analysis by inductively coupled plasma time-of-flight (TOF) technology, the basis of CyTOF® mass cytometers. A discrete signal from each ionized metal tag is detected based on differences in mass instead of wavelength, and at 1 Da resolution with minimal background.

What Antibodies are available for IMC?

Since 2018, we have developed a large inventory of metal tagged IMC antibodies. Some are purchased directly from Fluidigm but many more are conjugated at our laboratory using metal labelling kits manufactured by Fluidigm. Our inventory is continuously expanding. If we don’t stock your marker/clone we can custom conjugate. Please contact us for more details.

Please reach us at [email protected] for more information

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