Lunaphore COMET

Our industry leading spatial biology service suite now includes the COMET^™ platform to perform hyperplex assays based on flexible and fully automated sequential immunofluorescence (seqIF^™) to develop custom panels.

Capabilities

• Throughput – high capacity (4 slides) and rapid turnaround time (40 minutes every two markers) provide unmatched hyperplex throughput.
• Fast assay development: panels can be developed in weeks rather than months. Panels are optimized by characterizing simple modules of 1-2 markers. Once characterized, markers can be shuffled in any protocol cycle or added to any new panel without any further optimization. Batch optimization possible (Figure 2).
• Hyperplex: automated 40-plex runs detected on the same tissue slide.
• Reproducibility – full automation, use of standard primary antibodies without the need of complex upstream conjugation and direct detection via secondary antibodies, eliminate downstream complexity and variability leading to high reproducibility.
• Quality – highly controlled staining conditions thanks to a microfluidic technology (FFeX™) deliver immunofluorescence multiplex analysis comparable to classic IHC.
• Preserved tissue morphology – low exposure to reagents thanks to rapid incubations, and a gentle antibody removal, preserve the tissue to obtain high-quality stainings and enable downstream applications and H&E cross-validation on the same sample.
• Off-the-shelf primary antibodies – with a reagent agnostic approach, optimize using your own clones and reagent library; no conjugation and further validation required.
• Subcellular resolution – 230 nm resolution
• Integrated microscope – 20X magnification, TRITC, Cy5, DAPI channels.
• Common sample types: FFPE, FS, TMAs
• Output OME.TIFF – file supported by most image analysis tools.

Content

• Extensive database of panels as well as qualified and verified marker protocols