Immune Monitoring

Multiplex immunohistochemistry (mIHC) enables the identification of different immune cell subsets, their activation state, and their spatial distribution in the tumor microenvironment (TME) allowing maximum data collection from precious clinical samples. In practice, the simultaneous detection of multiple protein biomarkers on a single tissue section can be challenging therefore in a clinical setting it is essential to implement a rigorous optimization and validation process to ensure the assay can be trusted and is fit for purpose. Step 1Optimize the single IHC of each target. Identify antibody clone, antigen retrieval conditions and antibody incubation times for each target using positive control tissues. Multiplex immunohistochemistry (mIHC) enables the identification of different immune cell subsets, their activation state, and their spatial distribution in the tumor microenvironment (TME) allowing maximum data collection from precious clinicalsamples. In practice, the simultaneous detection of multiple protein biomarkers on a single tissue section can be challenging therefore in a clinical setting it is essential to implement a rigorous optimization and validation process to ensure the assay can be trusted and is fit for purpose. Step 1Optimize the single IHC of each target. Identify antibody clone, antigen retrieval conditions and antibody incubation times for each target using positive control […]